Rembrandt painted more than one version of the Genesis 22 story. The first, called The Sacrifice of Abraham, was painted around 1637. The second, entitled the Sacrifice of Isaac, is an etching done in 1655. In the time between these two depictions, Rembrandt's own son died. Follow these links to see the images:
The Sacrifice of Abraham
The Sacrifice of Isaac
The story told in Genesis 22 is known as the "Akedah" or "binding of Isaac". The philosophical, ethical and theological issues of this story have been tackled by many including: Kirkegaard, Levinas, Wolterstorff, and many many Jewish and Muslim scholars.